Platelet-derived mitochondria transfer accelerates fracture healing by promoting osteogenic differentiation and metabolic reprogramming of adipose-derived stem cells

Figure 4 The transfer of mitochondria to adipose-derived stem cells promotes histone lactylation. A: After mitochondria and adipose-derived stem cells (ASCs) were co-cultured in a series of ratios, the fluorescence intensity of mitochondrial-labeled ASCs was detected by flow cytometry; B: The linear relationship between mean fluorescence intensity value and mitochondria/ASCs ratio was analyzed using linear regression; C: In the untreated, platelet-treated and mitochondrial-treated groups of ASCs, mitochondria in ASCs were labeled with MitoTracker dye. 4’,6-diamidino-2-phenylindole was used to label the cell nuclei (200 × magnification); D: After treating with mitochondria, the levels of total lactylation (pan-kla), H3K27 lactylation (H3K27 La), H3K18 La, and H3K9 La were examined using western blotting. n = 3. Data were analyzed using Student’s t-test and are presented as mean ± SD. NS: Not significant; MFI: Mean fluorescence intensity; ASC: Adipose-derived stem cell; DAPI: 4’,6-diamidino-2-phenylindole.