miR-140-3p in exosomes derived from bone-marrow mesenchymal stem cells inhibits pyrotoisosis by targeting PTEN under high-glucose condition

Figure 5 Western blot results. A-E: Compared with the control group, bone-marrow mesenchymal stem cell secreted exosomes transfected with the inhibitor of miR-140-3p could significantly increase the expression of pyroptosis-related proteins gasdermine-D-N-terminal (GSDMD-N) (A), cleaved caspase-1 (B), interleukin (IL)-1β (C), IL-18 (D), and NLRP3 (E) in skin fibroblasts under high-glucose conditions. Adding the pyroptosis pathway inhibitor AYC to the Hy + miR-140-3p inhibitor-Exo group could significantly decrease the expression of pyroptosis-related proteins GSDMD-N (A), cleaved caspase-1 (B), IL-1β (C), IL-18 (D), and NLRP3 (E); F: The expression of GSDMD-N, cleaved caspase-1, IL-1β, IL-18, and NLRP3 in cells with different treatments was detected by western blot. n = 6. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. GSDMD-N: Gasdermine-D-N-terminal; IL: Interleukin.