Dysregulated circARF3/miR-27b-3p/YAP1 contributes to impaired osteogenic differentiation of periodontal ligament stem cells in periodontitis

Figure 5 miR-27b-3p targets the Yes associated protein 1 3’ untranslated region. A: Potential binding site of miR-27b-3p on Yes associated protein 1 (YAP1) 3’ untranslated region was predicted by ENCORI website; B: Luciferase activity of wild-type (WT) and mutated (MUT) YAP1 promoter gene vectors. ^bP < 0.01, compared with mimics NC group; C: Level of YAP1 in periodontal ligament stem cells after transfection with miR-27b-3p mimics was determined by quantitative real-time polymerase chain reaction. ^bP < 0.01, compared with mimics NC group; D: Level of YAP1 in periodontitis tissues and heathy samples. ^bP < 0.01, compared with healthy group; E and F: RNA and protein levels of YAP1 in periodontal ligament stem cells were determined by quantitative real-time polymerase chain reaction and western blotting assay, respectively. ^bP < 0.01, compared with shNC group; ^cP < 0.01, compared with shARF3 group. YAP1: Yes associated protein 1.