Dysregulated circARF3/miR-27b-3p/YAP1 contributes to impaired osteogenic differentiation of periodontal ligament stem cells in periodontitis

Figure 4 Circular RNA ADP-ribosylation factor 3 regulates periodontal ligament stem cell osteogenic differentiation via miR-27b-3p. A: Level of miR-27b-3p in periodontal ligament stem cells transfected with miR-27b-3p mimics or inhibitors. ^bP < 0.01, compared with inhibitor NC group; ^cP < 0.01, compared with mimics NC group; B and C: The expression of osteoblast markers Runx2, Osterix, Osteocalcin and Col1a1, was determined by western blotting assay. ^bP < 0.01, compared with inhibitor NC group; ^cP < 0.01, compared with mimics NC group; D: Alkaline phosphatase and alizarin red staining of periodontal ligament stem cells treated with miR-27b-3p mimics or inhibitors. ^bP < 0.01, compared with inhibitor NC group; ^cP < 0.01, compared with mimics NC group; E: Level of miR-27b-3p in periodontitis tissues and heathy samples. ^bP < 0.01, compared with healthy group. ARS: Alizarin red staining; ALP: Alkaline phosphatase.