Dysregulated circARF3/miR-27b-3p/YAP1 contributes to impaired osteogenic differentiation of periodontal ligament stem cells in periodontitis

Figure 3 Circular RNA ADP-ribosylation factor 3 acts as sponge for miR-27b-3p. A: Potential binding site of miR-27b-3p on circular RNA ADP-ribosylation factor 3 (circARF3) predicted by ENCORI website; B: Level of miR-27b-3p determined by quantitative real-time polymerase chain reaction. ^bP < 0.01, compared with mimic NC group; C: Luciferase activity of wild-type (WT) and mutated (MUT) circARF3 promoter gene vectors. ^bP < 0.01, compared with mimic NC group; D: RNA pulldown assay to check interaction between biotin-labeled WT and MUT miR-27b-3p and circARF3. ^bP < 0.01, compared with Bio-NC group; ^cP < 0.01, compared with Bio-miR-27b-3pWT group; E: RNA immunoprecipitation assay to check interaction between miR-27b-3p and circARF3. ^bP < 0.01, compared with IgG group; F: Level of miR-27b-3p in periodontal ligament stem cells after transfection with circARF3-overexpressing vectors determined by quantitative real-time polymerase chain reaction. ^bP < 0.01, compared with ovNC group. ARF3: ADP-ribosylation factor 3; WT: Wild-type; MUT: Mutated.