Dysregulated circARF3/miR-27b-3p/YAP1 contributes to impaired osteogenic differentiation of periodontal ligament stem cells in periodontitis

Figure 2 Circular RNA ADP-ribosylation factor 3 modulates osteogenic differentiation of periodontal ligament stem cells. A: Level of circular RNA ADP-ribosylation factor 3 (circARF3) in periodontal ligament stem cells treated with circARF3 overexpressing plasmids and small hairpin RNAs that target circARF3. ^bP < 0.01, compared with shNC group or ovNC group; B and C: Alkaline phosphatase and alizarin red staining of periodontal ligament stem cells treated with circARF3-overexpressing plasmids and small hairpin RNAs that target circARF3. ^bP < 0.01, compared with ovNC group; ^cP < 0.01, compared with shNC group; D: The expression of osteoblast makers, Runx2, Osterix, Osteocalcin and Col1a1, was determined by western blotting assay. ^bP < 0.01, compared with ovNC group; ^cP < 0.01, compared with shNC group. ARF3: ADP-ribosylation factor 3; ARS: Alizarin red staining; ALP: Alkaline phosphatase.