Hypoxic preconditioning restores the regenerative potential of aged adipose tissue derived mesenchymal stem cells

Figure 8 Representative micrographs of senescence-associated β-galactosidase staining. The images were captured at 10 × magnification. Arrow head represents senescence cells. A: Young adipose tissue derived mesenchymal stem cells (ADMSCs) displayed few senescence-associated β-galactosidase (SA-β-gal)-positive cells, indicating a low baseline level of senescence; B: Aged normoxia ADMSCs exhibited a markedly higher proportion of SA-β-gal-positive cells; C: Aged hypoxia ADMSCs demonstrated a visibly reduced proportion of SA-β-gal-positive cells compared with their normoxic aged counterparts.