Hypoxic preconditioning restores the regenerative potential of aged adipose tissue derived mesenchymal stem cells

Figure 2 Proliferative potential of adipose tissue derived mesenchymal stem cells under hypoxic condition. A and B: Cumulative population doublings (cPDs) and population doubling time (DT) was assessed in young adipose tissue derived mesenchymal stem cells (ADMSCs), aged normoxia ADMSCs and aged hypoxia ADMSCs group. Young ADMSCs exhibited significantly higher cPDs compared to aged normoxic ADMSCs, indicating decline in proliferative potential with aging. Aged hypoxia ADMSCs showed an increase in cPDs although the difference was not statistically significant. DT was significantly longer in aged normoxia ADMSCs as compared to young ADMSCs and Aged hypoxia ADMSCs. No significant difference in DT was observed between young and aged hypoxia ADMSCs that showed much youthful improvement in their proliferative potential. Data are presented as mean ± SD. ^bP < 0.01, ^dP < 0.0001. NS: Not significant; DT: Doubling time.