Hypoxia-conditioned extracellular vesicles from human amniotic membrane stem cells attenuate D-galactose-induced aging in mice

Table 1 Proteome analysis

Accession	Protein name	Gene name	#PSM	Ratio	UCBSC (reference)	Remark
P09486	SPARC	SPARC	349	2.24%	0.13%
P02452	Collagen alpha-1 (I) chain	COL1A1	312	2.00%	0.51%	Collagen
P02751-1	Isoform 1 of fibronectin	FN1	297	1.90%	3.32%	Cytoskeleton
P21333	Filamin-A	FLNA	264	1.69%	0.58%	Cytoskeleton
P08123	Collagen alpha-2 (I) chain	COL1A2	214	1.37%	0.23%	Collagen
P08670	Vimentin	VIM	207	1.33%	0.74%
P12111	Collagen alpha-3 (VI) chain	COL6A3	204	1.31%	2.31%	Collagen
P12109	Collagen alpha-1 (VI) chain	COL6A1	187	1.20%	0.56%	Collagen
P35555	Fibrillin-1	FBN1	180	1.15%	0.72%
P02461	Collagen alpha-1 (III) chain	COL3A1	168	1.08%	0.14%	Collagen
P60709	Actin, cytoplasmic 1	ACTB	154	0.99%	0.92%
Q99715	Collagen alpha-1 (XII) chain	COL12A1	150	0.96%	1.04%
Q15582	Transforming growth factor-beta-induced protein ig-h3	TGFBI	150	0.96%	0.66%
A0A804HK76	Filamin B	FLNB	149	0.95%	0.14%
A0A804HII9	Actinin alpha 1	ACTN1	134	0.86%	0.49%
O00469-2	Isoform 2 of procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2	PLOD2	131	0.84%	0.12%
P02787	Serotransferrin	TF	111	0.71%	ND	Iron transporter
O43707	Alpha-actinin-4	ACTN4	110	0.70%	0.50%
P04264	Keratin, type II cytoskeletal 1	KRT1	109	0.70%	0.25%
P68032	Actin, alpha cardiac muscle 1	ACTC1	107	0.69%	0.56%
P07585	Decorin	DCN	103	0.66%	0.32%
P01033	Metalloproteinase inhibitor 1	TIMP1	100	0.64%	0.03%	Collagenase inhibitor
P08253	72 kDa type IV collagenase	MMP2	99	0.63%	0.41%
A0A7P0TA35	Protein disulfide-isomerase	P4HB	97	0.62%	0.10%
P05121	Plasminogen activator inhibitor 1	SERPINE1	96	0.62%	0.36%
P26038	Moesin	MSN	94	0.60%	0.39%
P09871	Complement C1s subcomponent	C1S	91	0.58%	0.29%
Q12841	Follistatin-related protein 1	FSTL1	91	0.58%	0.06%
P35908	Keratin, type II cytoskeletal 2 epidermal	KRT2	90	0.58%	0.21%
A0A1B0GVI3	Keratin 10	KRT10	89	0.57%	0.34%
A0A7I2V3T0	CD9 molecule	CD9	16	0.02%	0.06%	Positive marker
F8VWK8	Tetraspanin (fragment)	CD63	11	0.03%	0.04%	Positive marker
H0YEE2	CD81 molecule	CD81	20	0.03%	0.07%	Positive marker

Top 30 proteins and exosome markers (CD9, CD63, and CD81) from total 751 proteins identified were listed. UCBSC: Umbilical cord blood stem cell; ND: Not detected.