Modulation of osteoarthritis-related microRNAs using locked nucleic acid-antisense oligonucleotides boosts chondrogenic lineage commitment of mesenchymal progenitors

Figure 5 Sulphated glycosaminoglycans content in day-21 micromasses. Figure shows a DMMB assay with chondroitin sulphate standards. Sulphated glycosaminoglycans values were normalised to dsDNA for each micromass (sulphated glycosaminoglycans/dsDNA). Increases were observed for miR-30b-5p alone and its combinations (miR-30b-5p/miR-16-5p, miR-30b-5p/miR-146a-5p, miR-30b-5p/miR-193b-3p), for miR-146a-5p/miR-193b-3p, and for the all-four mix; decreases were seen for miR-193b-3p alone and miR-16-5p/miR-193b-3p. Bars show mean ± SEM; n = 3; statistics vs power inhibitor control. Statistical comparisons vs power inhibitor by two-tailed Student’s t-test; ^bP < 0.01, ^cP < 0.005. sGAG: Sulphated glycosaminoglycan.