Adipose-derived stem cell stimulation using poly-L-lactic acid injection

Table 3 Experimental labeling parameters for primary antibodies at the respective validated concentrations and secondary labels for primary study

Primary antibody	Vendor and SKU	Primary concentration	Secondary antibody	Vendor and SKU	Secondary concentration
Panel 1
PCNA	Thermofisher, 60097-1-IG	1:500	Goat αMouse IgG1 488	Invitrogen, A21121	1:500
CD105	Thermofisher, 10862-1-AP	1:1000	Goat αRabbit 555	Invitrogen, A21428	1:500
CD90	Thermofisher, 66766-1-IG	1:250	Goat αMouse IgG2a 594	Invitrogen, A21135	1:500
CD31	Invitrogen, MA3105	15 μg/mL	Donkey αGoat 647	Invitrogen, A21447	1:500
Panel 2
β-catenin	Invitrogen, 13-8400	1:100	Goat αMouse IgG1 488	Invitrogen, A21121	1:500
Adiponectin	Thermofisher, TA804768	1:50	Goat αMouse IgG2b 594	Invitrogen, A21145	1:500
Pref-1	Thermofisher, 29288-1-AP	1:100	Goat αRabbit 647	Invitrogen, A21245	1:500
Panel 3
PLIN1	Cell Signaling Technology, 9349	1:100	Goat αRabbit 594	Invitrogen, A11012	1:500
Panel 4
PPAR-γ	Thermofisher, 66936-1-IG	1:250	Goat αMouse IgG1 647	Invitrogen, A21240	1:500

PCNA: Proliferating cell nuclear antigen; Pref-1: Preadipocyte factor 1; PLIN1: Perilipin 1; PPAR-γ: Peroxisome proliferator-activated receptor gamma; IgG: Immunoglobulin G.