Adipose-derived stem cell stimulation using poly-L-lactic acid injection

doi:10.4252/wjsc.v18.i4.116175

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Stem Cells. Apr 26, 2026; 18(4): 116175
Published online Apr 26, 2026. doi: 10.4252/wjsc.v18.i4.116175

Table 1 Material information for histological preparation, antibody validation and final labeling

	Material information
Glycine	Fisher scientific	212485
Normal donkey serum	Innovative Research, Inc.	IGDNSER500ML-36672
Goat serum	Gibco	16210064
TBS (10 ×), pH = 7.4	Quality Biological	351-086-491
TritonX-100	Thermo Scientific	A16046.AP
Xylene substitute-3	StatLab	7400-1
100% reagent alcohol	StatLab	6900-1
95% reagent alcohol	StatLab	9500-1
70% reagent alcohol type 1 water blend	StatLab	7070-1
Citraconic anhydride	Thermo Scientific Chemicals	L05238.14
PCNA monoclonal antibody (10D10E11)	Thermo Fisher Scientific	60097-1-IG
CD90 monoclonal antibody (2D7D11)	Thermo Fisher Scientific	66766-1-IG
Endoglin/CD105 polyclonal antibody	Thermo Fisher Scientific	10862-1-AP
CD31 monoclonal antibody (2H8)	Invitrogen	MA3105
Adiponectin monoclonal antibody (OTI3G10), TrueMAB™	Thermo Fisher Scientific	TA804768
DLK1 polyclonal antibody	Thermo Fisher Scientific	29288-1-AP
Β-catenin monoclonal antibody (CAT-5H10)	Invitrogen	13-8400
PPARγ monoclonal antibody (1F4A2)	Thermo Fisher Scientific	66936-1-IG
Perilipin-1 (D1D8) XP^® rabbit mAb #9349	Cell Signaling Technology	9349
Anti-CD68 antibody (EPR20545) - goat IgG (Chimeric)	Abcam	ab289671
Anti-CD80 antibody (RM1133)	Abcam	ab315832
CD163 recombinant rabbit monoclonal antibody (JA51-30)	Thermo Fisher Scientific	MA5-32684
DAPI	Invitrogen	D3571
Donkey αGoat 594	Invitrogen	A11058
Donkey αGoat 647	Invitrogen	A32849
Donkey αRabbit 647	Invitrogen	A31573
Goat αMouse IgG1 488	Invitrogen	A21121
Goat αMouse IgG1 647	Invitrogen	A21240
Goat αMouse IgG2a 594	Invitrogen	A21135
Goat αRabbit 555	Invitrogen	A21428
Goat αRabbit 594	Invitrogen	A11012
Goat αRabbit 647	Invitrogen	A21245
Fluoro-Gel (with Tris buffer)	Electron Microscopy Sciences	17985-10

TBS: Tris-buffered saline; PCNA: Proliferating cell nuclear antigen; DLK1: Delta-like non-canonical Notch ligand 1; PPAR-γ: Peroxisome proliferator-activated receptor gamma; IgG: Immunoglobulin G; DAPI: 4’,6-diamidino-2-phenylindole.

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Citation: Zubair RA, Zhou XH, Fabi SG. Adipose-derived stem cell stimulation using poly-L-lactic acid injection. World J Stem Cells 2026; 18(4): 116175
URL: https://www.wjgnet.com/1948-0210/full/v18/i4/116175.htm
DOI: https://dx.doi.org/10.4252/wjsc.v18.i4.116175

Zubair RA, Zhou XH, Fabi SG. Adipose-derived stem cell stimulation using poly-L-lactic acid injection. World J Stem Cells 2026; 18(4): 116175 [DOI: 10.4252/wjsc.v18.i4.116175]

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