WTAP-mediated N6-methyladenosine modification of circ_PVT1 promotes proliferation and tenogenic differentiation of tendon stem/progenitor cells

Figure 3 Knockdown of circular RNA plasmacytoma variant translocation 1 promoted oxidative stress and mitochondrial damage in transforming growth factor-β1-induced tendon stem/progenitor cells. A and B: The DCFH-DA assay was used to detect the content of reactive oxygen species (ROS) generation of tendon stem/progenitor cells (TSPCs) in the negative control (NC), transforming growth factor (TGF)-β1, TGF-β1 + shNC, and TGF-β1 + circular RNA plasmacytoma variant translocation 1 (circ_PVT1) groups; C-E: The detection of malondialdehyde concentration, glutathione concentration, and superoxide dismutase activity in TSPCs of the NC, TGF-β1, TGF-β1 + shNC, and TGF-β1 + circ_PVT1 groups; F and G: The JC-1 assay was performed to evaluate the mitochondrial membrane potential of TSPCs in the NC, TGF-β1, TGF-β1 + shNC, and TGF-β1 + circ_PVT1 groups; H: The ATP production of TSPCs in the NC, TGF-β1, TGF-β1 + shNC, and TGF-β1 + circ_PVT1 groups. All data are presented as the mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. NS: No significance; NC: Negative control; TGF-β1: Transforming growth factor-β1; circ_PVT1: Circular RNA plasmacytoma variant translocation 1; ROS: Reactive oxygen species; MDA: Malondialdehyde; GSH: Glutathione; SOD: Superoxide dismutase.