Maximizing pediatric stem cell harvest: A novel two-step digestion approach for adipose tissue

Figure 5 Mean gene expression of target genes. Mean gene expression of target genes normalized to GAPDH in adipose tissue (AT), AT1, AT2, stromal vascular fraction 1, stromal vascular fraction 2. A-H: Mean gene expression of vascular endothelial growth factor (A), stromal cell-derived factor (B), nitric oxide synthase-3 (C), CD14 (D), matrix metalloproteinase 3 (E), interleukin-4 receptor (F), P16 (G), and P53 (H). Differences between all fractions that are shown in the graphs of all genes are not significant statistically as P > 0.05. VEGF: Vascular endothelial growth factor; SDF: Stromal cell-derived factor; NOS3: Nitric oxide synthase-3; MMP3: Matrix metalloproteinase 3; IL4R: Interleukin-4 receptor; CDKN2A: Cyclin-dependent kinase inhibitor 2A; AT: Adipose tissue; SVF: Stromal vascular fraction.