Acupuncture improves transplanted neural stem cells in dementia mice by modulating hippocampal microenvironment via microRNA-124 mediated Notch and Wnt pathways

Figure 5 Expression of the interacted protein cyclin D1 and cell proliferation results. A: Cell cycle protein D1 flow cytometry results; B: Cell cycle protein D1 expression; C: Cell cycle flow cytometry results; D: Cell cycle proliferation index; E: G0/G1 results; F: G2/M results; G: S results; H: Nucleotides percentage; I: Cell apoptosis flow cytometry results; J: Apoptosis rate. ^aP < 0.05, compared with the senescence-accelerated mice resistant 1 control group; ^bP < 0.05, compared with the senescence-accelerated mouse prone 8 control group; ^cP < 0.05, compared with the senescence-accelerated mouse prone 8-sham transplantation group; ^dP < 0.05, compared with the senescence-accelerated mouse prone 8-neural stem cells transplantation with non-acupoint group; ^eP < 0.05, compared with the senescence-accelerated mouse prone 8-neural stem cells transplantation with acupoint and microRNA-124 low expression group. RC: Senescence-accelerated mice resistant 1 control group; PC: Senescence-accelerated mouse prone 8 control group; PT: Senescence-accelerated mouse prone 8-neural stem cells transplantation group; PS: Senescence-accelerated mouse prone 8-sham transplantation group; PTA: Senescence-accelerated mouse prone 8-neural stem cells transplantation with acupoint group; PTN: Senescence-accelerated mouse prone 8-neural stem cells transplantation with non-acupoint group; PTAH: Senescence-accelerated mouse prone 8-neural stem cells transplantation with acupoint and microRNA-124 high expression group; PTAL: Senescence-accelerated mouse prone 8-neural stem cells transplantation with acupoint and microRNA-124 low expression group; PTAC: Senescence-accelerated mouse prone 8-neural stem cells transplantation with acupoint and microRNA-124 control group.