Acupuncture improves transplanted neural stem cells in dementia mice by modulating hippocampal microenvironment via microRNA-124 mediated Notch and Wnt pathways

Figure 3 Results of the water maze hidden platform test, Alzheimer’s disease pathological marker expression, and microRNA-124 gene expression. A-C: Water maze test results. The time from entering the water to finding the platform was recorded as the escape latency. If the mice did not find the platform within 90 seconds, the escape latency was recorded as 90 seconds; D-G: Alzheimer’s disease pathological marker expression; H: MicroRNA-124 gene expression. ^aP < 0.05, compared with the senescence-accelerated mice resistant 1 control group; ^bP < 0.05, compared with the senescence-accelerated mouse prone 8 control group; ^cP < 0.05, compared with the senescence-accelerated mouse prone 8-sham transplantation group; ^dP < 0.05, compared with the senescence-accelerated mouse prone 8-neural stem cells transplantation with non-acupoint group; ^eP < 0.05, compared with the senescence-accelerated mouse prone 8-neural stem cells transplantation with acupoint and microRNA-124 low expression group. RC: Senescence-accelerated mice resistant 1 control group; PC: Senescence-accelerated mouse prone 8 control group; PT: Senescence-accelerated mouse prone 8-neural stem cells transplantation group; PS: Senescence-accelerated mouse prone 8-sham transplantation group; PTA: Senescence-accelerated mouse prone 8-neural stem cells transplantation with acupoint group; PTN: Senescence-accelerated mouse prone 8-neural stem cells transplantation with non-acupoint group; PTAH: Senescence-accelerated mouse prone 8-neural stem cells transplantation with acupoint and microRNA-124 high expression group; PTAL: Senescence-accelerated mouse prone 8-neural stem cells transplantation with acupoint and microRNA-124 low expression group; PTAC: Senescence-accelerated mouse prone 8-neural stem cells transplantation with acupoint and microRNA-124 control group; Aβ: Amyloid β; APP: Amyloid precursor protein; P-Tau: Phosphorylated tau.