Human amniotic mesenchymal stem cell-derived small extracellular vesicles repair of jaw osteoradionecrosis

Figure 6 Human amniotic mesenchymal stem cell-derived small extracellular vesicles activated Wnt/β-catenin expression in irradiated human umbilical vein endothelial cells. A: Kyoto Encyclopedia of Genes and Genomes pathway analysis illustrating the top 30 downregulated genes in human umbilical vein endothelial cells (HUVECs) between the 12 Gy and control groups; B: Kyoto Encyclopedia of Genes and Genomes pathway analysis illustrating the top 30 upregulated genes in HUVECs between the 12 Gy + 10¹⁰ human amniotic mesenchymal stem cell-derived small extracellular vesicles (hAMSCs-sEVs) and 12 Gy groups; C-E: Western blot and polymerase chain reaction analysis of β-catenin expression in HUVECs in the control, 12 Gy and 12 Gy + 10¹⁰ hAMSCs-sEVs groups; F-I: Western blot and polymerase chain reaction analysis of β-catenin and vascular endothelial growth factor expression in HUVECs in the control, 12 Gy and 12 Gy + 10¹⁰ hAMSCs-sEVs groups treated with or without the selective inhibitor MSAB; J-L: Tube formation assay in the control, 12 Gy and 12 Gy + 10¹⁰ hAMSCs-sEVs groups treated with or without the selective inhibitor MSAB. Scale bar = 100 μm; M and N: Transwell assay in the control, 12 Gy and 12 Gy + 10¹⁰ hAMSCs-sEVs groups treated with or without the selective inhibitor MSAB. Scale bar = 100 μm; O and P: Scratch wound healing assay in the control, 12 Gy and 12 Gy + 10¹⁰ hAMSCs-sEVs groups treated with or without the selective inhibitor MSAB. ^aP < 0.05, ^bP < 0.01. VEGF: Vascular endothelial growth factor; sEVs: Small extracellular vesicles.