Allogeneic myoblast cell sheet transplantation for esophageal wall reinforcement

Figure 6 Ki-67-positive cells in the transplanted myoblast cell sheets at each time point. A and B: Ki-67 immunostaining (× 400, scale bar: 100 μm) of the transplantation group at 1 week postoperatively showed that the layer formed by the myoblast cell sheet contained numerous Ki-67-positive cells (A), and 4 weeks postoperatively showed very few Ki-67 positive cells (proliferating cells) in the transplanted myoblast sheet layer (enclosed by a dotted line) (B); C: The number of Ki-67-positive cells per high-power field (× 400) detected via Ki-67 immunostaining showed that Ki-67-positive cells in the myoblast sheet-transplanted area at 4 weeks post-transplantation were significantly reduced compared to that at 1 week post-transplantation.