Allogeneic myoblast cell sheet transplantation for esophageal wall reinforcement

Figure 4 Histological evaluation at 1 week postoperatively. A: Histological examination of the control group [hematoxylin and eosin (HE) staining, × 40] revealed no tissue replacement at the site of the esophageal muscularis propria resection. Fibrous adhesion was noted between the resected area and the liver (△); B: HE staining (× 40) of the transplantation group showed the myoblast cell sheet proliferating and replacing the resected esophageal muscularis propria (▲); C: HE staining (× 400, scale bar: 100 μm) of the transplantation group showed the cells in the myoblast sheet had partially formed a multinucleated myotube (△); D: Desmin staining (× 400, scale bar: 100 μm) of the transplantation group showed that the myoblast cell sheet contained fibrous desmin-positive cells, indicating proliferation of the myoblast cell sheet.