GATA2 deficiency exacerbates chronic liver injury via disrupting hepatocyte death-regeneration balance: Clinical, histopathological, and molecular evidence

Figure 8 Hepatocyte-specific Gata2 knockdown exacerbates hepatocyte death-regeneration imbalance in chronic liver injury mice. A-C: Gata2 shRNA-transfected healthy mice: Serum alanine aminotransferase levels (A); hepatic histopathology by hematoxylin and eosin staining (B); western blot analysis of GATA2 protein expression (representative blots shown) with quantification (C); D-I: Gata2 knockdown intervention in chronic liver injury mice: Effect of Gata2 knockdown on serum alanine aminotransferase levels in chronic liver injury mice (D); serum total bilirubin levels (E); quantitative analysis of necrotic areas with representative hematoxylin and eosin and Masson’s trichrome staining images (F); fluorescent Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining analysis of hepatocyte apoptosis rate (representative images shown) (G); ferrous ion content (H); western blot analysis of GATA2 protein expression (blots displayed) with quantification (I); J: Western blot analysis of proliferating cell nuclear antigen, cleaved caspase-3, and phosphorylated mixed lineage kinase domain-like protein expression (representative blots shown) with quantification. ns: P > 0.05, ^bP < 0.01 vs control group; ^cP < 0.05 vs chronic liver injury group (CCl₄), ^dP < 0.01 vs chronic liver injury group (CCl₄). ALT: Alanine aminotransferase; H&E: Hematoxylin and eosin; TBil: Total bilirubin; PCNA: Proliferating cell nuclear antigen; p-MLKL: Phosphorylated mixed lineage kinase domain-like protein; MLKL: Mixed lineage kinase domain-like protein.