GATA2 deficiency exacerbates chronic liver injury via disrupting hepatocyte death-regeneration balance: Clinical, histopathological, and molecular evidence

Figure 7 GATA2 overexpression protected HepG2 cells from endoplasmic reticulum stress-induced injury. A: Fluorescence microscopy images confirming transfection efficiency; B: Western blot analysis of GATA2 protein expression, with representative blots displayed alongside quantitative results; C: Assessment of cell viability using the Cell Counting Kit-8 assay; D: Intracellular malondialdehyde content; E: Glutathione levels; F: Oxidized glutathione levels; G: Electron transfer chain complex I activity; H: Triglyceride levels; I: Ferrous ion levels; J: Western blot analysis of proliferating cell nuclear antigen, cleaved caspase-3, and phosphorylated mixed lineage kinase domain-like protein expression (representative blots shown) with quantification. ^cP < 0.05 vs the thapsigargin group; ^dP < 0.01 vs the thapsigargin group. THA: Thapsigargin; MDA: Malondialdehyde; GSH: Glutathione; GSSG: Oxidized glutathione; ETC-CI: Electron transfer chain complex I; TG: Triglyceride; p-MLKL: Phosphorylated mixed lineage kinase domain-like protein; MLKL: Mixed lineage kinase domain-like protein.