GATA2 deficiency exacerbates chronic liver injury via disrupting hepatocyte death-regeneration balance: Clinical, histopathological, and molecular evidence

Figure 4 Identification of GATA2 binding sites in gene promoters through chromatin immunoprecipitation sequencing and prediction of its regulatory functions. A: Scatter plot showing the correlation between biological replicates; B: Histogram depicting the distribution of peak lengths; C: Distribution of peaks across chromosomes (X-axis: Chromosome length; left Y-axis: Peak intensity; right Y-axis: Chromosome numbers); D: Read distribution surrounding transcription start sites (TSS) of peak-associated genes (top: Line chart of average sequencing depth around TSS; bottom: Heatmap of sequencing depth around TSS for peak-associated genes, ranked by average depth); E: Gene Ontology functional enrichment analysis of peak-associated genes; F: Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of peak-associated genes; G: Fold enrichment of selected genes associated with partial regeneration, molecular chaperones, antioxidant response, and fatty acid β-oxidation as determined by chromatin immunoprecipitation sequencing; H: Validation of GATA2 binding to the promoters of heat shock protein family D member 1, glutathione peroxidase 4, and peroxisome proliferator-activated receptor alpha by chromatin immunoprecipitation real-time quantitative polymerase chain reaction. Data are presented as fold enrichment relative to immunoglobulin G; I: Western blot analysis of GATA2, heat shock protein 60, glutathione peroxidase 4, and peroxisome proliferator-activated receptor alpha protein expression following transfection with the Gata2 construct. ^bP < 0.01 vs immunoglobulin G or control group. ChIP-seq: Chromatin immunoprecipitation sequencing; IgG: Immunoglobulin G; PCNA: Proliferating cell nuclear antigen; HSPD1: Heat shock protein family D member 1; GPX4: Glutathione peroxidase 4; UCP2: Uncoupling protein 2; PPARA: Peroxisome proliferator-activated receptor alpha.