Soluble immunosuppressive checkpointome of mesenchymal stromal cells: Immunoregulatory mechanisms and a conceptual framework for potency assessment

Figure 4 Proposed translational workflow for a checkpoint-based potency assay presents a hypothetical development framework rather than a validated clinical workflow. It illustrates the experimental and analytical steps that would need to be evaluated before a checkpoint-based composite score could be considered for donor screening, batch qualification or patient stratification. No element of this workflow is validated; it defines future validation studies rather than a usable clinical procedure. IFN-γ: Interferon gamma; TNF-α: Tumor necrosis factor alpha.