Aging puzzle: A closer look on the complex dilemma of autologous stem cell therapy

Figure 6 Cryopreservation of cells at slow freezing and rapid cooling. This figure illustrates the two main cryopreservation techniques, slow freezing and rapid cooling. In the slow freezing method, cells are incubated in a cryoprotectant medium with low concentration, leading to some cell damage and extracellular ice formation. In contrast, rapid cooling involves the use of a cryoprotectant medium with high concentration, preserving cell integrity and preventing the formation of ice crystals within the cells.