Hypoxia facilitates triple-negative breast cancer stem cells enrichment and stemness maintenance through oxidized ataxia telangiectasia mutated-induced one-carbon metabolism

Figure 2 Oxidized ataxia telangiectasia mutated promotes triple-negative breast cancer-cancer stem cells enrichment and stemness maintenance. A: Representative images showing inhibition of mammosphere formation by the ataxia telangiectasia mutated inhibitor Ku60019; B: Quantification of mammosphere size; C: Quantification of mammosphere number; D-F: Effects of ataxia telangiectasia mutated knockdown on cancer stem cell enrichment (D), mammosphere size (E), and mammosphere number (F); G and H: Western blot analysis of stemness-associated proteins (c-Myc, sex-determining region Y-box 2, Kruppel-like factor 4, octamer-binding protein 4) following Ku60019 treatment (G) or ataxia telangiectasia mutated knockdown (H). Scale bar: 200 μm. Data were presented as mean ± SD (n = 3). ^aP < 0.05; ^bP < 0.01. KLF4: Kruppel-like factor 4; SOX2: Sex-determining region Y-box 2; p-ATM: Phosphorylated ataxia telangiectasia mutated; shATM: Ataxia telangiectasia mutated knockdown.