Quercetin pretreated umbilical cord mesenchymal stem cells alleviate inflammatory bowel disease via IL-10 /Janus kinase 2/STAT3 signaling

Figure 5 Colon tissue RNA sequencing: Transcriptomic analysis of colon tissue in an acute colitis model to investigate differential gene expression at transcriptional levels. A: Differentially gene expression (DEG) volcano map. 2,4,6-trinitrobenzenesulfonic acid (TNBS) vs quercetin (QUR)-human umbilical cord-derived mesenchymal stem cells (hUCMSCs); B: DEG volcano map. hUCMSCs vs QUR-hUCMSCs. The red dots represent upregulated genes, whereas the green dots represent downregulated genes; C: Hierarchical clustering analysis of DEGs; D: Gene Ontology enrichment analysis. The -log10(P-value) is used to measure the expression of DEGs; E: Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway. Point size indicates the number of genes in the pathway, and the point color corresponds to P-adjusted ranges; F: Protein network interaction analysis. Nodes represent proteins, and the connections between nodes represent the strength of protein-protein interactions. Target symbol size was related to the degree value; among them, the larger the target’s shape, the more critical the target in the network. BP: Biological processes; CC: Cellular component; JAK: Janus kinase; MF: Molecular function; STAT: Signal transducer and activator of transcription; Th17: T helper type 17.