Stem cell-derived neural organoids as platforms to investigate glioblastoma invasion and migration: A systematic review

Table 2 Characteristics of neural organoid models

Ref.	Cell/stem cell	iPSCs source	Cell number	NOs age (days)	Assessment size	Characterization1
Van De Looverbosch et al[33]	hiPSCs (iPSC0028)	Epithelium	10000	30	1077 ± 272 μm	NR
Ferreira et al[34]	hiPSCs (F9048)	Skin fibroblasts	10000	40	NR	SOX2, TUJ1, DCX
Pedrosa et al[25]	hiPSCs (BJiPSC-SV4F-9)	Skin fibroblasts	NR	41	NR	SOX2, TUJ1, GFAP, O4
Fedorova et al[35]	hiPSCs (MUNIi008-A)	Skin fibroblasts	2000-3000	55	NR	PAX6, TUJ1, DCX, MAP2, BRN2, SYN1
	hiPSCs (MUNIi009-A)
	hiPSCs (MUNIi010-A)
Bassot et al[36]	hiPSCs + hESCs (HS420)	Fetal skin fibroblasts + Blastocyst	1000	40	NR	TUJ1, MAP2, NeuN, GFAP, S100β, OLIG2
Goranci-Buzhala et al[37]	hiPSCs (IMR90)	Fetal lung fibroblast	35000	10	NR	NR
Azzarelli et al[14]	hiPSCs (IMR90)	Fetal lung fibroblast	9000	42	NR	SOX2, N-cadherin, PAX6, TUJ1, TBR1
Krieger et al[38]	hiPSCs (409b2)	Skin fibroblasts	1000	24	500-900 μm	PAX6, TUJ1
Goranci-Buzhala et al[23]	hiPSC (IMR90) + GSCs	Fetal lung fibroblast	35000 (hiPSC) + 1000 (GSC)	20, 40 and 60	500-700 μm	MAP2, SYN1
			35000 (hiPSC) + 5000 (GSC)
Linkous et al[24]	hESCs (WA01 and WA09)	Blastocyst	9000	NR	NR	Nestin, musashi-1, SOX2, PAX6 and TBR2
	hiPSCs (H6)	NR

¹Were considered only positive markers.

iPSCs: Induced pluripotent stem cells; NOs: Neural organoids; hiPSCs: Human induced pluripotent stem cells; NR: No reported; SOX2: Sex determining region box 2; TUJ1: Beta-tubulin III; DCX: Doublecortin; GFAP: Glial fibrillary acidic protein; PAX6: Paired box protein 6; BRN2: BEARSKIN2; SNY1: Synapsin1; hESCs: Human embryonic stem cells; MAP2: Microtubule-associated protein 2; NeuN: Neuron-specific nuclear; OLIG2: Oligodendrocyte transcription factor 2; S100β: Calcium-binding protein β; GSCs: Glioblastoma stem cells.