X inactive-specific transcript regulates mitochondrial function and neuronal differentiation of stem cells via IGF2BP2/CPT1A axis in models of spinal cord injury

Figure 2 X inactive-specific transcript enhanced neural stem cell proliferation and influenced cell differentiation. A: CCK-8 assay showed the proliferation of neural stem cells transfected with X inactive-specific transcript (XIST) compared with the negative control (NC) at days 1, 3, and 7; B: Relative expression of the neuronal marker microtubule-associated protein 2 at day 7, showing upregulation in the XIST group compared to NC; C: Relative expression of the astrocytic marker glial fibrillary acidic protein at day 7, showing downregulation in the XIST group compared to NC. Data are presented as mean ± SD. ^aP < 0.01, ^bP < 0.001. XIST: X inactive-specific transcript; NC: Negative control; MAP2: Microtubule-associated protein 2; GFAP: Glial fibrillary acidic protein.