X inactive-specific transcript regulates mitochondrial function and neuronal differentiation of stem cells via IGF2BP2/CPT1A axis in models of spinal cord injury

Figure 1 X inactive-specific transcript overexpression enhanced mitochondrial function in neural stem cells. A: Relative X inactive-specific transcript (XIST) expression level measured by PCR, indicating successful overexpression of XIST in neural stem cells; B: JC-1 staining assay showing the relative JC-1 intensity, reflecting mitochondrial membrane potential; C: Relative mitochondrial DNA copy number assessed by PCR, indicating an increase in mitochondrial biogenesis; D: Relative ATP production levels measured using an ATP assay kit, demonstrating enhanced ATP generation; E: Oxygen consumption rate result showed increased mitochondrial respiration in XIST-overexpressing neural stem cells. Data are presented as mean ± SD. ^aP < 0.01, ^bP < 0.001. XIST: X inactive-specific transcript; NC: Negative control; mtDNA: Mitochondrial DNA; OCR: Oxygen consumption rate; FCCP: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone.