Hypoxic preconditioned mesenchymal stem cell-derived exosomes alleviate oxidative stress-induced cardiomyocyte apoptosis through miR-486-5p

Figure 5 Potential signaling pathway and target genes involved in the protective effects of exosomal miR-486-5p. A: Kyoto Encyclopedia of Genes and Genomes analyses showed the top 20 significantly enriched signaling pathways of miR-486-5p; B: Venn diagram illustrates the number of targets of miR-486-5p among three bioinformatics platforms; C: MiR-486-5p regulated phosphatase and tensin homolog (PTEN) by directly targeting the 3’ untranslated region (UTR); D: Relative luciferase activity was measured in HEK293T cells transfected with luciferase reporter vectors containing either the wild-type or mutant binding site of miR-486-5p in the 3’ UTR of PTEN, along with a miR-486-5p mimic or negative control (n = 3). ^bP < 0.01.