Hypoxic preconditioned mesenchymal stem cell-derived exosomes alleviate oxidative stress-induced cardiomyocyte apoptosis through miR-486-5p

Figure 4 miR-486-5p mediated the cell protective effects of hypoxia-preconditioned mesenchymal stem cell-derived exosomes. A: Heat map based on high-throughput microRNAs sequencing representing the 25 identified microRNAs with the most significant abundance differences between normoxic counterparts (normo-Exos) and hypoxia-preconditioned mesenchymal stem cell-derived exosomes (hypo-Exos); B: Volcano plot showing log2 (fold change) on the X-axis and -log10 (P value) on the Y-axis; C: MiR-486-5p expression level in normo-Exos and hypo-Exos determined by quantitative polymerase chain reaction; D: MiR-486-5p antagomir or negative control transfected into hypo-Exos altered the miR-486-5p expression level in hypo-Exos; E: Expression of apoptosis-related protein was determined by western blotting; F-H: Quantitative analysis of the western blotting; I: Level of apoptosis was determined by flow cytometry; J: Statistical evaluation of flow cytometry. All data are representative of three independent experiments and are presented as the mean ± standard error of the mean (n = 3). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001.