Hypoxic preconditioned mesenchymal stem cell-derived exosomes alleviate oxidative stress-induced cardiomyocyte apoptosis through miR-486-5p

Figure 3 Human umbilical cord-derived mesenchymal stem cells exosomes ameliorated oxidative stress-induced apoptosis in H9C2 cells. A: Different effects of normoxic counterparts (normo-Exos) and hypoxia-preconditioned mesenchymal stem cell-derived exosomes (hypo-Exos) on the viability of H9C2 cells; B: Different effects of normo-Exos and hypo-Exos on superoxide dismutase (SOD) activity in hydrogen peroxide (H₂O₂)-induced H9C2 cells; C: Different effects of normo-Exos and hypo-Exos on lactate dehydrogenase (LDH) release in H₂O₂-induced H9C2 cells; D: Expression of apoptosis-related protein was determined by western blotting; E-G: Quantitative analysis of the western blotting; H: Level of apoptosis was determined by flow cytometry; I: Statistical evaluation of flow cytometry. Data are presented as the mean ± standard error of the mean (n = 3). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001.