Isolation and characterization of CD73+CD39+CD146+ mesenchymal stem cell subset from bone marrow

Figure 2 Sorting strategy and purity post-sort for bone marrow-derived CD45-CD73+CD39+ subset. A: Sorting strategy for bone marrow (BM)-derived mesenchymal stem cells (MSCs) for first sorting (a1 and a2). Cell population was defined based on cell size, granularity, and single cells. Cells were gated on the viable population (a3). The cell fraction negative for CD45 was gated (a4) and cells which co-expressed CD73 and CD39 were gated and isolated based on single stained controls (a5). Controls not shown; B: Sorting strategy for BM-derived MSCs for second sort (b1 and b2). Cell population was defined based on cell size, granularity, and single cells. Cells were gated on the viable population (b3). The cell fraction negative for CD45 was gated (b4) and cells which co-expressed CD73 and CD39 were gated and isolated based on single stained controls (b5). Controls not shown; C: Bar graphs showing percentage of the CD73+CD39+ cell subset in samples obtained post-sorting.