Impact of differentiation protocols on the functionality of mesenchymal stem cells derived from induced pluripotent stem cells

Figure 4 Analysis of cell viability, mitochondrial membrane potential, and intracellular reactive oxygen species cellular for induced pluripotent stem cells-derived mesenchymal stem cell lines. A: Absorbance results of MTT assay showing viable cell numbers in induced pluripotent stem cells-derived mesenchymal stem cell (iMSC) lines compared to bone marrow mesenchymal stem cells at 24 hours and 48 hours after cell seeding; B: Percentage of colony-forming units among iMSC lines compared to bone marrow mesenchymal stem cells (control); C: Red/green fluorescence ratio indicating mitochondrial membrane potential in untreated and carbonyl cyanide 3-chlorophenylhydrazone-treated iMSC samples; D: Fluorescent intensities of total reactive oxygen species production in iMSC lines. Tert-butyl hydroperoxide was used as a positive control to induce reactive oxygen species production. ^bP ≤ 0.01; ^cP ≤ 0.001; ^dP ≤ 0.0001. BM-MSCs: Bone marrow mesenchymal stem cells; EB-iMSCs: Embryoid body-induced pluripotent stem cells-derived mesenchymal stem cells; iMSC: Induced pluripotent stem cells-derived mesenchymal stem cell; CFUs: Colony-forming units; MMP: Mitochondrial membrane potential; CCCP: Carbonyl cyanide 3-chlorophenylhydrazone; TBHP: Tert-butyl hydroperoxide; ROS: Reactive oxygen species.