Impact of differentiation protocols on the functionality of mesenchymal stem cells derived from induced pluripotent stem cells

doi:10.4252/wjsc.v17.i12.110564

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Dec 26, 2025; 17(12): 110564
Published online Dec 26, 2025. doi: 10.4252/wjsc.v17.i12.110564

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Figure 1 Flow cytometry analysis of pluripotency cell surface markers in induced pluripotent stem cells-derived mesenchymal stem cell lines generated using different protocols. A: Representative microscopic images showing the morphology of induced pluripotent stem cells (iPSCs)-derived mesenchymal stem cell (iMSC) lines compared to bone marrow mesenchymal stem cells (BM-MSCs), scale bar = 100 μm; B: Flow cytometry histograms of pluripotency markers NANOG and TRA-1-60 in iMSC lines compared to iPSCs (positive control) and BM-MSCs (negative control); C: Bar graph displaying the percentages of pluripotency markers in iMSC lines relative to control cells (BM-MSCs and iPSCs). Data represent mean ± SE from at least three independent technical replicates. BM-MSCs: Bone marrow mesenchymal stem cells; EB-iMSCs: Embryoid body-induced pluripotent stem cells-derived mesenchymal stem cells; iMSC: Induced pluripotent stem cells-derived mesenchymal stem cell; iPSCs: Induced pluripotent stem cells.

Citation: Ababneh NA, Alwohoush E, AlDiqs R, Ismail MA, Al-Kurdi B, Barham R, Al-Atoom R, Nairat F, Al Hadidi S, Whaibi S, Gharandouq MH, Zalloum S, Al Shboul S, Al-Qaisi T, Abuhammad A, Saleh T, Awidi A. Impact of differentiation protocols on the functionality of mesenchymal stem cells derived from induced pluripotent stem cells. World J Stem Cells 2025; 17(12): 110564
URL: https://www.wjgnet.com/1948-0210/full/v17/i12/110564.htm
DOI: https://dx.doi.org/10.4252/wjsc.v17.i12.110564

Ababneh NA, Alwohoush E, AlDiqs R, Ismail MA, Al-Kurdi B, Barham R, Al-Atoom R, Nairat F, Al Hadidi S, Whaibi S, Gharandouq MH, Zalloum S, Al Shboul S, Al-Qaisi T, Abuhammad A, Saleh T, Awidi A. Impact of differentiation protocols on the functionality of mesenchymal stem cells derived from induced pluripotent stem cells. World J Stem Cells 2025; 17(12): 110564 [PMID: 41480400 DOI: 10.4252/wjsc.v17.i12.110564]

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