HIF-1α modulates β-catenin pathway to enhance the survival and angiogenesis of PBMSCs under hypoxia environment

Figure 6 β-catenin regulates expressions of antiapoptotic and proangiogenic molecules. The experiments were categorized into the following groups: Transfection of vehicle in normoxia (normoxia-Con), transfection of vehicle in hypoxia (hypoxia-Con), β-catenin overexpression, β-catenin knockdown, and β-catenin overexpression plus β-catenin knockdown in hypoxia. A: The protein levels of vascular endothelial growth factor, basic fibroblast growth factor, survivin, Bcl2, Bax, and cleaved caspase 3 in peripheral blood mesenchymal stem cells were measured by western blot; B: The mRNA levels of survivin, Bcl2, and Bax in peripheral blood mesenchymal stem cells were measured by quantitative real-time polymerase chain reaction. All graphical data are presented as the means ± SEMs. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. n = 3 in each group. Normoxia-Con: Transfection of vehicle in normoxia; Hypoxia-Con: Transfection of vehicle in hypoxia; oeβ-catenin: Β-catenin overexpression; shβ-catenin: Β-catenin knockdown; oe + shβ-catenin: Β-catenin overexpression plus β-catenin knockdown in hypoxia; VEGF: Vascular endothelial growth factor; bFGF: Basic fibroblast growth factor.