HIF-1α modulates β-catenin pathway to enhance the survival and angiogenesis of PBMSCs under hypoxia environment

Figure 5 Hypoxia-inducible factor-1α regulates expressions of antiapoptotic and proangiogenic molecules. The experiments were categorized into the following groups: Transfection of vehicle in normoxia, transfection of vehicle in hypoxia, hypoxia-inducible factor-1α (HIF-1α) overexpression, HIF-1α knockdown, and HIF-1α overexpression plus HIF-1α knockdown in hypoxia. A: The protein levels of vascular endothelial growth factor, basic fibroblast growth factor, survivin, Bcl2, Bax, and cleaved caspase 3 in the peripheral blood mesenchymal stem cells were evaluated using western blotting; B: The mRNA levels of survivin, Bcl2, and Bax were detected using quantitative real-time polymerase chain reaction. All graphical data are presented as the means ± SEMs. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. n = 3 in each group. Normoxia-Con: Transfection of vehicle in normoxia; Hypoxia-Con: Transfection of vehicle in hypoxia; oeHIF-1α: Hypoxia-inducible factor-1α overexpression; shHIF-1α: Hypoxia-inducible factor-1α knockdown; oe + shHIF-1α: Hypoxia-inducible factor-1α overexpression plus hypoxia-inducible factor-1α knockdown in hypoxia; VEGF: Vascular endothelial growth factor; bFGF: Basic fibroblast growth factor.