HIF-1α modulates β-catenin pathway to enhance the survival and angiogenesis of PBMSCs under hypoxia environment

Figure 2 Hypoxia-inducible factor-1α promotes stem cell proliferation and inhibits the senescence of peripheral blood mesenchymal stem cells. The experiments were categorized into the following groups: Transfection of vehicle in normoxia, transfection of vehicle in hypoxia, hypoxia-inducible factor-1α (HIF-1α) overexpression, HIF-1α knockdown, and HIF-1α overexpression plus HIF-1α knockdown in hypoxia. A: Cell proliferative capacity of peripheral blood mesenchymal stem cells was assessed by cell colony formation assays and crystal violet staining; B: Quantitative analysis of the number of cell clones in each group; C: Β-galactosidase staining was employed to examine cellular senescence of peripheral blood mesenchymal stem cells; D: Quantitative analysis of percentage of β-galactosidase positive cells (scale bars = 50 μm). All graphical data are presented as the means ± SEMs. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. n = 3 in each group. Normoxia-Con: Transfection of vehicle in normoxia; Hypoxia-Con: Transfection of vehicle in hypoxia; oeHIF-1α: Hypoxia-inducible factor-1α overexpression; shHIF-1α: Hypoxia-inducible factor-1α knockdown; oe + shHIF-1α: Hypoxia-inducible factor-1α overexpression plus hypoxia-inducible factor-1α knockdown in hypoxia.