Sclerostin-silenced human umbilical cord mesenchymal stem cells ameliorate bone metabolism in steroid-induced femoral head necrosis

Figure 8 Effect of sclerostin inhibitor-silenced human umbilical cord mesenchymal stem cells on the expression of bone metabolic factors in the bone marrow of mice with steroid-induced avascular necrosis of the femoral head. A: Western bolt original picture; B-D: The expression level of alkaline phosphatase, β-catenin and osteoprotegerin were the lowest in the SAN group, the highest in the sham group, and the expression level of alkaline phosphatase, β-catenin and osteoprotegerin in the sh-MSCs group was less than that in the MSCs group; E: The expression level of sclerostin was the lowest in the sham group, the highest in the SAN group, and the expression level of sclerostin in the sh-MSCs group was less than that in the MSCs group. Data represent mean ± SEM from three independent experiments. ^bP < 0.01 vs sham; ^cP < 0.05 vs SAN; ^dP < 0.01 vs SAN. ALP: Alkaline phosphatase; OPG: Osteoprotegerin; SOST: Sclerostin; GADPH: Glyceraldehyde-3-phosphate dehydrogenase.