RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

Table 1 Candidate reference genes for quantitative real-time polymerase chain reaction

Gene symbol	Function	NCBI access	Primers sequences (5’-3’)	Temperature (°C)
RPLP0	Component of the 60S subunit	NM_001002	F: AGCCCAGAACACTGGTCTC	60
			R: ACTCAGGATTTCAATGGTGCC
TBP	Transcription, metabolic pathways	NM_003194.5	F: CACGAACCACGGCACTGATT	62
			R: TTTTCTTGCTGCCAGTCTGGA
YWHAZ	Signal transduction	NM_001135702	F: TGATCCCCAATGCTTCACAAG	61
			R: GCCAAGTAACGGTAGTAATCTCC
RUNX2	Master transcription factor for osteogenesis	NM_001015051	F: TGGTTACTGTCATGGCGGGTA	62
			R: TCTCAGATCGTTGAACCTTGCTA
EF1α	Translation	NM_001402	F: GAAGCTGGTATCTCCAAGAATGG	61
			R: CGACAATTAGTTGTTTCACACCC
GAPDH	Mitochondrial metabolism	NM_002046	F: GCATCCTGGGCTACACTGA	60
			R: CCACCACCCTGTTGCTGTA
ACTB	Cytoskeleton	NM_001101	F: CGACAGGATGCAGAAGGAG	60
			R: TCCTGCTTGCTGATCCACAT
B2M	Immune response	NM_004048	F: GAGGCTATCCAGCGTACTCCA	62
			R: CGGCAGGCATACTCATCTTTT
ALAS1	Mitochondrial metabolism	NM_199166	F: AAATGAATGCCGTGAGGAAAGA	60
			R: CCCTCCATCGGTTTTCACACTA
TUB	Cytoskeleton	NM_001293212	F: TCAACACCTTCTTCAGTGAAACG	60
			R: AGTGCCAGTGCGAACTTCATC
SDHA	Mitochondrial metabolism	NM_004168	F: CAAACAGGAACCCGAGGTTTT	60
			R: CAGCTTGGTAACACATGCTGTAT

ACTB: Actin beta; ALAS1: Aminolevulinic acid synthase 1; B2M: Beta-2-microglobulin; EF1α: Eukaryotic translational elongation factor 1 alpha; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NCBI: National Cancer for Biotechnology Information: RPLP0: Ribosomal protein, large, P0; RUNX2: Runt-related transcription factor 2; SDHA: Succinate dehydrogenase complex, subunit A, flavoprotein; TBP: TATA-binding protein; TUB: Tubulin; YWHAZ: Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta.