Gossypol acetic acid regulates leukemia stem cells by degrading LRPPRC via inhibiting IL-6/JAK1/STAT3 signaling or resulting mitochondrial dysfunction

Figure 8 Gossypol acetic acid induced reactive oxygen species, disturbed mitochondrial homeostasis and resulted in mitochondrial dysfunction. A: Under normoxia and hypoxia conditions, after being treated with gossypol acetic acid (GAA) or S3I-210 for 24 h, cell viability was measured by performing CCK-8 assay. ^aP < 0.05, vs mock group; B: After being treated with or without GAA, cells were stained using H2DCFDA and detected by performing flow cytometry; C: After being cultured by GAA under hypoxia condition, mitochondrial homeostasis was measured by performing JC-1 staining. JC-1 FITC proportion was considered as disturbed mitochondrial homeostasis; D-F: After being cultured by GAA or S3I-210, LDH leakage was detected to measure cellular membrane impact (D); total ATP synthesis (E) and mitochondrial DNA mass (F) were measured to confirm the effects of GAA on mitochondrial function. ^aP < 0.05, vs mock group.