Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

Figure 10  Cell cycle analysis of recultured human umbilical cord-mesenchymal stem cells. A: The effect of recultured human umbilical cord-mesenchymal stem cells (hUC-MSCs) on cell cycle progression: The cell cycle progression was analyzed by flow cytometry. The histogram showed decrease in G₀/G1 phase of the cell cycle between recultured groups 1-4, 5-7, and 8-10. In contrast, no difference was observed in S phase of cell cycle among all three recultured hUC groups. However, the gradual increase in the percentages of hUC-MSCs present in G2/M phase among all three recultured groups (1-4, 5-7, and 8-10) was observed; B: The effects of recultured hUC-MSCs on cell cycle phases: The quantification of cellular percentages in the G0/G1, S and G2/M phases of cell cycle between each recultured groups was evaluated by flow cytometry. The result showed significant decrease in G₀/G1 phase of cell cycle at recultured numbers 1-4, 5-7, and 8-10. Conversely, significant increase in G2/M phase at recultured 1-4, 5-7, and 8-10 numbers of hUC-MSCs was observed. The cells percentage showed no significant difference in S phase of the cell cycle at all recultured groups; C: The effects of recultured hUC-MSCs on cell cycle progression: The effects of recultured hUC-MSC showed significantly increased G2/M phase of cell cycle at recultured 5-7 and 8-10. At the same time, results showed significantly decreased G0/G1 phase at recultured numbers 8-10 of hUC-MSCs. The data from six independent experiments is shown as mean ± SD with significant P-values. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001. The graphical representation shows cumulative data from six representative hUC samples analyzed. hUC: Human umbilical cord; MSC: Mesenchymal stem cell.