Expansion of human umbilical cord derived mesenchymal stem cells in regenerative medicine

Figure 8 Passaging and population doubling analysis of recultured human umbilical cord-mesenchymal stem cells. A: Microscopic morphology of recultured human umbilical cord-mesenchymal stem cells (hUC-MSCs) at passage 1-15: The hUC-MSCs cultured in minimal essential medium alpha at a seeding density of 1000 cells/cm². The representative images were captured at 80%-90% confluency. Experiments were performed in duplicates; B: The comparative effect of population doubling time with passage at every reculture number of hUC-MSC. The data of six independent experiments shows mean ± SD with significant P-values. ^aP < 0.05; C: The comparative population doubling time on recultured hUC number on respective passage number. The heat map for multiple comparisons between recultured hUC for each MSC passage number showed no significant effect on population doubling time; D: The effect of recultured hUC and MSCs passaging on population doubling number. hUC: Human umbilical cord; MSC: Mesenchymal stem cell.