Unveiling the role of hypoxia-inducible factor 2alpha in osteoporosis: Implications for bone health

Figure 5 Adult Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice exhibit normal hematopoiesis under different stimulation conditions. A-I: Eight-week-old Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice were subjected to semilethal irradiation. The white blood cell count (A), red blood cell count (B), hemoglobin content (C) and platelet count (D) in peripheral blood were determined. Four weeks after semilethal irradiation, bone marrow (BM) cells freshly harvested from 8-wk-old Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice were assayed by multiparameter fluorescence-activated cell sorting (FACS) analyses to determine the total nucleated cell (TNC) numbers (E), frequencies of hematopoietic stem cells (HSCs) (F), HSC numbers (G), frequencies of progenitors (H), and progenitor numbers (I); 8-wk-old Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice were treated with 5-fluorouracil (5-FU); J-N: Four weeks after the 5-FU treatment regimen, BM cells freshly harvested from 8-wk-old Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice were assayed via multiparameter FACS analyses to determine the TNC numbers (J), frequencies of HSCs (K), HSC numbers (L), frequencies of progenitors (M), and progenitor numbers (N). ^aP < 0.05. WBC: White blood cell; NEU: Neutrophil; HGB: Hemoglobin; PLT: Platelet; TNC: Total nucleated cell; HSC: Hematopoietic stem cell; LT-HSC: Long-term hematopoietic stem cell; ST-HSC: Short-term hematopoietic stem cell; MPP: Multipotent progenitor; CMP: Common myeloid progenitor; GMP: Granulocyte-monocyte progenitor; MEP: Megakaryocyte-erythroid progenitor; CLP: Common lymphoid progenitor; HIF-2α: Hypoxia-inducible factor 2alpha.