Unveiling the role of hypoxia-inducible factor 2alpha in osteoporosis: Implications for bone health

Figure 3 Adult Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice exhibit no difference in bone mass under the naive condition. A: Microcomputed tomography (μCT) reconstruction images of distal femoral metaphyses from 2-month-old Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice; B: Hematoxylin and eosin (HE) staining images of distal femoral metaphyses from 2-month-old Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice; C-K: Quantitative μCT analyses of the structural parameters of femoral trabeculae from 2-month-old mice: bone volume/tissue volume (C), trabecular number (D), trabecular thickness (E), and trabecular spacing (F); quantitative HE staining analysis of the structural parameters of femoral trabeculae from 2-month-old mice: bone volume/tissue volume (G), trabecular number (H), and trabecular thickness (I); bone marrow cells freshly harvested from 8-wk-old Prx1-Cre;Hif-2α^fl/fl and Hif-2α^fl/fl mice were assayed by multiparameter fluorescence-activated cell sorting analyses to determine the frequency (J) and number (K) of bone mesenchymal stem cells. ^aP < 0.05. MSC: Mesenchymal stem cell.