Single cell RNA sequencing reveals mesenchymal heterogeneity and critical functions of Cd271 in tooth development

Figure 6 Inference, analysis and visualization of cellular communication networks from a single dataset via CellChat. A: Interaction intensity plot between cells in single cell sequence profiling; B: Interaction intensity map between mesenchymal stem cells (MSCs) and other cell types; C: Contribution map of 30 signaling pathways detected by CellChat to intercellular efferent (or afferent) signaling; D: Alluvial plot of cell outgoing signaling patterns, showing the correspondence between cell populations and signaling pathways; E: Important ligand–receptor pairs for MSCs sending signals to other cell types. F and G: Contribution of efferent (or afferent) signals between cells, the MK signaling pathway (F) and PTN signaling pathway (G) were selected for visualization of network centrality scores; H: Expression of Mdk and Ptn in Cd271 knockout and wild-type MSCs was analyzed by quantitative real-time polymerase chain reaction. Data are presented as mean values ± SD (n = 3 biologically independent experiments. Two-sided unpaired t-test, ^aP < 0.05, ^bP < 0.01). KO: Knockout; WT: Wild-type.