Single cell RNA sequencing reveals mesenchymal heterogeneity and critical functions of Cd271 in tooth development

Figure 1 Single cell RNA sequencing analysis of maxillary processes from wild-type and Cd271 knockout mice. We identified 21 clusters of cells, and the single cell RNA sequencing data were characterized. A: Schematic representation of the experimental workflow. The Cd271 knockout and wild-type maxillary processes were collected from mouse embryos at 16.5 d. After genotyping, cells were suspended as single cells, and the cDNA library was constructed and sequenced; B: Cells extracted from the samples were subjected to Uniform Manifold Approximation and Projection hierarchical clustering and color-coded by cluster grouping, predominant cell type, and sample origin; C: Heatmap showing expression levels of differentially expressed genes in each cluster; D: Dot plot showing prominent marker genes for each cell type (mesenchymal cells, epithelial, muscle cell, macrophage, glial, T cell, endothelial, and perivascular); E: Pie chart showing the number of each cell type; F: Histogram showing the number and proportion of cells from wild-type and Cd271 knockout mice. UMAP: Uniform Manifold Approximation and Projection; KO: Knockout; WT: Wild-type.