Dissecting molecular mechanisms underlying ferroptosis in human umbilical cord mesenchymal stem cells: Role of cystathionine γ-lyase/hydrogen sulfide pathway

Figure 2 Ferroptosis exists in human umbilical cord mesenchymal stem cells. A-C: Cell viability was assessed after exposure to different concentrations of erastin for different times (12 h, 24 h, and 48 h); D: Cell viability was assessed after exposure to 3 μM erastin and different concentrations of ferrostatin-1 (Fer-1). Cell viability was measured with Cell Counting Kit-8 kit; E-G: Lactic dehydrogenase (LDH) release was assessed after exposure to different concentrations of erastin for different times (12 h, 24 h, and 48 h); H: LDH release was assessed after exposure to 3 μM erastin and different concentrations of Fer-1. LDH release was measured with LDH cytotoxicity detection kit. The data were from at least three independent experiments. Data were quantified for cells subjected to erastin (0 μM), and values are represented as the mean ± SD. Fer-1: Ferrostatin-1; LDH: Lactic dehydrogenase. ^bP < 0.01.