MicroRNA-584-5p/RUNX family transcription factor 2 axis mediates hypoxia-induced osteogenic differentiation of periosteal stem cells

Figure 5 MiRNA-584-5p regulates hypoxia-induced osteogenic differentiation of periosteal stem cells by targeting RUNX family transcription factor 2. A: Alkaline phosphatase activity; B: Intracellular calcium ion level; C: Alizarin red S staining chelated Ca²⁺ form an orange-red complex; D: Osteogenic differentiation-related protein expression. One-way ANOVA and t tests were used to demonstrate differences between the miR-581-5p mimic + pcDNA3.1-, NC mimic-, and miR-584-59 mimic + pc-RUNX family transcription factor 2-expressing constructs. ^aP < 0.05; ^bP < 0.01. ALP: Alkaline phosphatase; RUNX2: RUNX family transcription factor 2; BMP2: Bone morphogenetic protein 2; HIF-1α: Hypoxia-inducible factor 1; miR: MicroRNA; NC: Negative control.