MicroRNA-584-5p/RUNX family transcription factor 2 axis mediates hypoxia-induced osteogenic differentiation of periosteal stem cells

Figure 4 Downregulation of RUNX family transcription factor 2 inhibits the osteogenic differentiation of periosteal stem cells induced by hypoxia. A: RUNX family transcription factor 2 (RUNX2) expression; B: Alkaline phosphatase activity; C: Intracellular calcium ion level; D: Alizarin red S staining chelated Ca²⁺ form an orange-red complex; E: Osteogenic differentiation-related protein expression. One-way ANOVA and t tests were used to demonstrate differences between the sh-RUNX2 and normal control shRNA groups. ^aP < 0.05; ^bP < 0.01. ALP: Alkaline phosphatase; RUNX2: RUNX family transcription factor 2; BMP2: Bone morphogenetic protein 2; HIF-1α: Hypoxia-inducible factor 1; shRNA: Small hairpin RNA.